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  • Featured Product
  • KD/KO Validated

CYB5R3 Monoclonal antibody

CYB5R3 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

9C4D10

Cat No : 66022-1-Ig

Print datasheet

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

验证数据展示

  • WB analysis of HeLa using 66022-1-Ig

    WB analysis of HeLa using 66022-1-Ig

    WB results of CYB5R3 antibody (66022-1-Ig, 1:60000) with si-Control and si-CYB5R3 transfected HeLa cells.

  • IHC staining of human liver cancer using 66022-1-Ig

    IHC staining of human liver cancer using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IHC staining of human liver cancer using 66022-1-Ig

    IHC staining of human liver cancer using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IF Staining of HeLa using 66022-1-Ig

    IF Staining of HeLa using 66022-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of HeLa using 66022-1-Ig

    WB analysis of HeLa using 66022-1-Ig

    Western blot analysis of CYB5R3-specific in various tissues using Proteintech antibody 66022-1-Ig at dilution of 1:40000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, human heart tissue, human plasma tissue, HepG2 cells, L02 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CYB5R3 Ready-To-Use IHC Kit. CYB5R3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66022-1-Ig targets CYB5R3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA Application Description
Tested Reactivity human
Immunogen CYB5R3 fusion protein Ag18096 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cytochrome b5 reductase 3
Synonyms B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC004821
Gene Symbol CYB5R3
Gene ID (NCBI) 1727
RRIDAB_11045662
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00387
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

实验方案

Product Specific Protocols
WB protocol for CYB5R3 antibody 66022-1-IgDownload protocol
IHC protocol for CYB5R3 antibody 66022-1-IgDownload protocol
IF protocol for CYB5R3 antibody 66022-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols