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验证数据展示

  • IF Staining of HepG2 using CL488-66022

    IF Staining of HepG2 using CL488-66022

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66022 (CYB5R3 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CYB5R3 Monoclonal antibody

CYB5R3 Monoclonal Antibody for IF/ICC
Cat No. CL488-66022
CloneNo. 9C4D10

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CYB5R3 Ready-To-Use IHC Kit. CYB5R3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66022-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66022 targets CYB5R3 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CYB5R3 fusion protein Ag18096 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cytochrome b5 reductase 3
Synonyms B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC004821
Gene Symbol CYB5R3
Gene ID (NCBI) 1727
RRIDAB_2883230
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00387
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CYB5R3 antibody CL488-66022Download protocol
Standard Protocols
Click here to view our Standard Protocols