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验证数据展示

  • FC experiment of HepG2 using CL647-13241

    FC experiment of HepG2 using CL647-13241

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated CYP1A1 Polyclonal antibody (CL647-13241)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated CYP1A1 Polyclonal antibody

CYP1A1 Polyclonal Antibody for FC (Intra)
Cat No. CL647-13241

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, pig

应用

FC (Intra)

别名

Cytochrome P450 1A1, CYPIA1, CYP1, CP11, AHRR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CYP1A1 Ready-To-Use IHC Kit. CYP1A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13241-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-13241 targets CYP1A1 in FC (Intra) applications and shows reactivity with human, mouse, pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, pig
免疫原 CYP1A1 fusion protein Ag3990 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 1, subfamily A, polypeptide 1
别名 Cytochrome P450 1A1, CYPIA1, CYP1, CP11, AHRR
计算分子量 512 aa, 58 kDa
观测分子量 50-58 kDa
GenBank蛋白编号BC023019
基因名称 CYP1A1
Gene ID (NCBI) 1543
RRIDAB_2934882
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04798
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CYP1A1 antibody CL647-13241Download protocol
Standard Protocols
Click here to view our Standard Protocols