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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated CYP1A1 Polyclonal antibody

CYP1A1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13241

Print datasheet

Synonyms

Cytochrome P450 1A1, CYPIA1, CYP1, CP11, AHRR

验证数据展示

  • FC experiment of HepG2 using CL647-13241

    FC experiment of HepG2 using CL647-13241

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated CYP1A1 Polyclonal antibody (CL647-13241)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CYP1A1 Ready-To-Use IHC Kit. CYP1A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13241-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-13241 targets CYP1A1 in FC (Intra) applications and shows reactivity with human, mouse, pig samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, pig
Immunogen CYP1A1 fusion protein Ag3990 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 1, subfamily A, polypeptide 1
Synonyms Cytochrome P450 1A1, CYPIA1, CYP1, CP11, AHRR
Calculated Molecular Weight 512 aa, 58 kDa
Observed Molecular Weight 50-58 kDa
GenBank Accession NumberBC023019
Gene Symbol CYP1A1
Gene ID (NCBI) 1543
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04798
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CYP1A1 antibody CL647-13241Download protocol
Standard Protocols
Click here to view our Standard Protocols