验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, PC-3 cells, rat brain tissue, mouse brain tissue, pig brain tissue, T-47D cells, MCF-7 cells |
| Positive IHC detected in | mouse heart tissue, human rectal cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CYP1B1 Ready-To-Use IHC Kit. CYP1B1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67033-1-Ig targets CYP1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | CYP1B1 fusion protein Ag13380 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 1, subfamily B, polypeptide 1 |
| 别名 | GLC3A, EC:4.2.1.152, EC:1.14.14.1, CYPIB1, CP1B |
| 计算分子量 | 61 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC012049 |
| 基因名称 | CYP1B1 |
| Gene ID (NCBI) | 1545 |
| RRID | AB_2882348 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16678 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP1B1 antibody 67033-1-Ig | Download protocol |
| IHC protocol for CYP1B1 antibody 67033-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
