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验证数据展示

  • WB analysis using 67033-1-Ig (same clone as 67033-1-PBS)

    WB analysis using 67033-1-Ig (same clone as 67033-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

    IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP1B1 antibody included
  • IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

    IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP1B1 antibody included

CYP1B1 Monoclonal antibody, PBS Only

CYP1B1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67033-1-PBS

产品说明书

CloneNo. 1G8D4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Indirect ELISA

别名

GLC3A, EC:4.2.1.152, EC:1.14.14.1, CYPIB1, CP1B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CYP1B1 Ready-To-Use IHC Kit. CYP1B1 primary antibody included.

产品信息

67033-1-PBS targets CYP1B1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 CYP1B1 fusion protein Ag13380 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cytochrome P450, family 1, subfamily B, polypeptide 1
别名 GLC3A, EC:4.2.1.152, EC:1.14.14.1, CYPIB1, CP1B
计算分子量 61 kDa
观测分子量 52 kDa
GenBank蛋白编号BC012049
基因名称 CYP1B1
Gene ID (NCBI) 1545
RRIDAB_2882348
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16678
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).