• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CYP1B1 Monoclonal antibody, PBS Only

CYP1B1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G8D4

Cat no : 67033-1-PBS

Print datasheet

Synonyms

GLC3A, EC:4.2.1.152, EC:1.14.14.1, CYPIB1, CP1B

验证数据展示

  • WB analysis using 67033-1-Ig (same clone as 67033-1-PBS)

    WB analysis using 67033-1-Ig (same clone as 67033-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

    IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP1B1 antibody included
  • IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

    IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP1B1 antibody included
Planning an IHC experiment? We recommend our IHCeasy CYP1B1 Ready-To-Use IHC Kit. CYP1B1 primary antibody included.

产品信息

67033-1-PBS targets CYP1B1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CYP1B1 fusion protein Ag13380 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytochrome P450, family 1, subfamily B, polypeptide 1
Synonyms GLC3A, EC:4.2.1.152, EC:1.14.14.1, CYPIB1, CP1B
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC012049
Gene Symbol CYP1B1
Gene ID (NCBI) 1545
RRIDAB_2882348
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16678
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).