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CoraLite® Plus 488-conjugated CYP20A1 Monoclonal antibody

CYP20A1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A9E2

Cat no : CL488-67433

Print datasheet

Synonyms

UNQ667/PRO1301, EC:1.14.-.-, Cytochrome P450 20A1, CYP M, 2A9E2

验证数据展示

  • IF Staining of HeLa using CL488-67433

    IF Staining of HeLa using CL488-67433

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CYP20A1 antibody (CL488-67433, Clone: 2A9E2 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67433

    FC experiment of HeLa using CL488-67433

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CYP20A1 Monoclonal antibody (CL488-67433, Clone:2A9E2)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67433-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67433 targets CYP20A1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CYP20A1 fusion protein Ag9715 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytochrome P450, family 20, subfamily A, polypeptide 1
Synonyms UNQ667/PRO1301, EC:1.14.-.-, Cytochrome P450 20A1, CYP M, 2A9E2
Calculated Molecular Weight 462 aa, 52 kDa
Observed Molecular Weight 49-52 kDa
GenBank Accession NumberBC020616
Gene Symbol CYP20A1
Gene ID (NCBI) 57404
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6UW02
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CYP20A1 antibody CL488-67433Download protocol
FC protocol for CL Plus 488 CYP20A1 antibody CL488-67433Download protocol
Standard Protocols
Click here to view our Standard Protocols