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验证数据展示

  • WB analysis using 84699-1-RR (same clone as 84699-1-PBS)

    WB analysis using 84699-1-RR (same clone as 84699-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84699-1-RR (CYP26A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84699-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 84699-1-RR (same clone as 84699-1-PBS)

    IHC staining of mouse skeletal muscle using 84699-1-RR (same clone as 84699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84699-1-RR (CYP26A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84699-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 84699-1-RR (same clone as 84699-1-PBS)

    IHC staining of mouse skeletal muscle using 84699-1-RR (same clone as 84699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84699-1-RR (CYP26A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84699-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 84699-1-RR (same clone as 84699-1-PBS)

    IF Staining of human placenta using 84699-1-RR (same clone as 84699-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CYP26A1 antibody (84699-1-RR, Clone: 241816D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84699-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84699-1-RR (same clone as 84699-1-PBS)

    FC experiment of HepG2 using 84699-1-RR (same clone as 84699-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CYP26A1 Recombinant antibody (84699-1-RR, Clone:241816D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84699-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84699-1-RR

    Affinity and Kinetic Characterization of 84699-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84699-1-RR against Human CYP26A1 were performed. The affinity constant is below 1 pM.

CYP26A1 Recombinant antibody, PBS Only

CYP26A1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 84699-1-PBS

产品说明书

CloneNo. 241816D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

Cytochrome P450RAI, Cytochrome P450 26A1, CYP26, CP26, 241816D11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84699-1-PBS targets CYP26A1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CYP26A1 fusion protein Ag24898 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome P450, family 26, subfamily A, polypeptide 1
别名 Cytochrome P450RAI, Cytochrome P450 26A1, CYP26, CP26, 241816D11
计算分子量 497 aa, 56 kDa
观测分子量 50-56 kDa
GenBank蛋白编号BC157063
基因名称 CYP26A1
Gene ID (NCBI) 1592
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO43174
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYP26A1 (Cytochrome P450 Family 26 Subfamily A Member 1), also known as Cytochrome P450 26A1, CP26, CYP26, P450RAI, and P450RAI1, is a member of the cytochrome P450 superfamily of enzymes, involved in retinoic acid (RA) metabolism and synthesis of cholesterol, steroids, and other lipids. CYP26A1 is essential for normal hindbrain patterning, vertebral identity, and development of posterior structures(PMID: 11157778).