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CYP27A1 Polyclonal antibody, PBS Only

CYP27A1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14739-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of mouse liver using 14739-1-AP (same clone as 14739-1-PBS)

    WB analysis of mouse liver using 14739-1-AP (same clone as 14739-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

    IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP27A1 antibody included
  • IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

    IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP27A1 antibody included
Planning an IHC experiment? We recommend our IHCeasy CYP27A1 Ready-To-Use IHC Kit. CYP27A1 primary antibody included.

产品信息

14739-1-PBS targets CYP27A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CYP27A1 fusion protein Ag6408 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 27, subfamily A, polypeptide 1
Synonyms
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC051851
Gene Symbol CYP27A1
Gene ID (NCBI) 1593
RRIDAB_2089276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02318
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.