CYP2A6 Polyclonal antibody
CYP2A6 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HepG2 cells, mouse lung tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
The immunogen of 21721-1-AP is CYP2A6 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CYP2A6 fusion protein Ag16377 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cytochrome P450, family 2, subfamily A, polypeptide 6 |
| Synonyms | Coumarin 7 hydroxylase, CYP2A, CYP2A3, CYP2A6, CYPIIA6, Cytochrome P450 2A6, Cytochrome P450 IIA3, Cytochrome P450(I), P450C2A, P450PB |
| Calculated Molecular Weight | 494 aa, 57 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC096253 |
| Gene Symbol | CYP2A6 |
| Gene ID (NCBI) | 1548 |
| RRID | AB_10791886 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11509 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP2A6 antibody 21721-1-AP | Download protocol |
| IHC protocol for CYP2A6 antibody 21721-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells. | ||
Xenobiotica Comparison of mouse and human cytochrome P450 mediated-drug metabolising activities in hepatic and extrahepatic microsomes. | ||
Sci Rep Extracellular vesicles released from macrophages modulates interleukin-1β in astrocytic and neuronal cells | ||
Front Toxicol E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis | ||
Biochem Biophys Res Commun Circadian rhythms in CYP2A5 expression underlie the time-dependent effect of tegafur on breast cancer | ||
Biochem Biophys Res Commun Circadian rhythms in CYP2A5 expression underlie the time-dependent effect of tegafur on breast cancer |
