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验证数据展示

  • IHC staining of human liver cancer using 60297-1-Ig

    IHC staining of human liver cancer using 60297-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60297-1-Ig (CYP2D6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYP2D6 antibody included
  • IHC staining of human liver cancer using 60297-1-Ig

    IHC staining of human liver cancer using 60297-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60297-1-Ig (CYP2D6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYP2D6 antibody included
  • WB analysis of HepG2 using 60297-1-Ig

    WB analysis of HepG2 using 60297-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60297-1-Ig (CYP2D6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CYP2D6 Monoclonal antibody

CYP2D6 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60297-1-Ig

产品说明书

CloneNo. 8B11D12

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, ELISA

别名

CPD6, CYP2D, CYP2D@, CYP2D6, CYP2DL1, Cytochrome P450 2D6, P450 DB1, P450C2D, P450DB1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, fetal human brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CYP2D6 Ready-To-Use IHC Kit. CYP2D6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60297-1-Ig targets CYP2D6 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 CYP2D6 fusion protein Ag12605 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cytochrome P450, family 2, subfamily D, polypeptide 6
别名 CPD6, CYP2D, CYP2D@, CYP2D6, CYP2DL1, Cytochrome P450 2D6, P450 DB1, P450C2D, P450DB1
计算分子量 497 aa, 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC075023
基因名称 CYP2D6
Gene ID (NCBI) 1565
RRIDAB_2881412
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10635
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP2D6(Cytochrome P450 2D6) is also named as CYP2DL1 and belongs to the cytochrome P450 family. It is a monooxygenase that involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. CYP2D6 is responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It has 2 isoforms produced by alternative splicing with the molecular weight of 56 kDa and 50 kDa.

实验方案

Product Specific Protocols
WB protocol for CYP2D6 antibody 60297-1-IgDownload protocol
IHC protocol for CYP2D6 antibody 60297-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols