CYP2E1-Specific Monoclonal antibody
CYP2E1-Specific Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
mouse, rat, pig, human
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1F12G7
验证数据展示
经过测试的应用
Positive WB detected in | pig liver tissue, rat liver tissue, mouse liver tissue |
Positive IHC detected in | human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse liver tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
IHC | See 1 publications below |
产品信息
67263-1-Ig targets CYP2E1-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat, pig, human samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | mouse, rat, pig, human |
Cited Reactivity | mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | cytochrome P450, family 2, subfamily E, polypeptide 1 |
Synonyms | 4 nitrophenol 2 hydroxylase, CPE1, CYP2E, CYP2E1, CYP2E1-Specific, CYPIIE1, Cytochrome P450 2E1, Cytochrome P450 J, P450 J, P450C2E |
Calculated Molecular Weight | 57 kDa |
Observed Molecular Weight | 50-57 kDa |
GenBank Accession Number | NM_000773 |
Gene Symbol | CYP2E1 |
Gene ID (NCBI) | 1571 |
RRID | AB_2882534 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P05181 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CYP2E1-Specific antibody 67263-1-Ig | Download protocol |
IHC protocol for CYP2E1-Specific antibody 67263-1-Ig | Download protocol |
IF protocol for CYP2E1-Specific antibody 67263-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biomed Pharmacother The effects of scoparone on alcohol and high-fat diet-induced liver injury revealed by RNA sequencing | ||
Front Pharmacol Methyl 6-O-cinnamoyl-α-d-glucopyranoside Ameliorates Acute Liver Injury by Inhibiting Oxidative Stress Through the Activation of Nrf2 Signaling Pathway. | ||
Med Sci Monit Comparison of the Effects of Intraperitoneal Injection with Carbon Tetrachloride on Acute Liver Toxicity in Male and Female Kunming Mice | ||
Nutrients Daucosterol Alleviates Alcohol−Induced Hepatic Injury and Inflammation through P38/NF−κB/NLRP3 Inflammasome Pathway |