CoraLite® Plus 488-conjugated CYP2E1-Specific Monoclonal antibody
CYP2E1-Specific Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
mouse, rat, pig, human
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F12G7
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse liver tissue |
Planning an IHC experiment? We recommend our IHCeasy CYP2E1 Ready-To-Use IHC Kit. CYP2E1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67263-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67263 targets CYP2E1-Specific in IF-P applications and shows reactivity with mouse, rat, pig, human samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | mouse, rat, pig, human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cytochrome P450, family 2, subfamily E, polypeptide 1 |
| Synonyms | 4 nitrophenol 2 hydroxylase, CPE1, CYP2E, CYP2E1, CYP2E1-Specific, CYPIIE1, Cytochrome P450 2E1, Cytochrome P450 J, P450 J, P450C2E |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 50-57 kDa |
| GenBank Accession Number | NM_000773 |
| Gene Symbol | CYP2E1 |
| Gene ID (NCBI) | 1571 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05181 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CYP2E1-Specific antibody CL488-67263 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |