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验证数据展示

  • IF Staining of mouse liver using CL488-67263

    IF Staining of mouse liver using CL488-67263

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 CYP2E1-Specific antibody (CL488-67263, Clone: 1F12G7 ) at dilution of 1:200.

  • IF Staining of mouse liver using CL488-67263

    IF Staining of mouse liver using CL488-67263

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 CYP2E1-Specific antibody (CL488-67263, Clone: 1F12G7 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CYP2E1-Specific Monoclonal antibody

CYP2E1-Specific Monoclonal Antibody for IF-P
Cat No. CL488-67263

产品说明书

CloneNo. 1F12G7

宿主/亚型

Mouse / IgG2b

种属反应性

mouse, rat, pig, human

应用

IF-P

别名

4 nitrophenol 2 hydroxylase, CPE1, CYP2E, CYP2E1, CYP2E1-Specific, CYPIIE1, Cytochrome P450 2E1, Cytochrome P450 J, P450 J, P450C2E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy CYP2E1 Ready-To-Use IHC Kit. CYP2E1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67263-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67263 targets CYP2E1-Specific in IF-P applications and shows reactivity with mouse, rat, pig, human samples.

经测试应用 IF-P Application Description
经测试反应性 mouse, rat, pig, human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cytochrome P450, family 2, subfamily E, polypeptide 1
别名 4 nitrophenol 2 hydroxylase, CPE1, CYP2E, CYP2E1, CYP2E1-Specific, CYPIIE1, Cytochrome P450 2E1, Cytochrome P450 J, P450 J, P450C2E
计算分子量 57 kDa
观测分子量 50-57 kDa
GenBank蛋白编号NM_000773
基因名称 CYP2E1
Gene ID (NCBI) 1571
RRIDAB_2934529
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05181
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CYP2E1-Specific antibody CL488-67263Download protocol
Standard Protocols
Click here to view our Standard Protocols