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验证数据展示

  • WB analysis using 68198-1-Ig (same clone as 68198-1-PBS)

    WB analysis using 68198-1-Ig (same clone as 68198-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68198-1-Ig (CYP2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68198-1-PBS in a different storage buffer formulation.

  • WB analysis using 68198-1-Ig (same clone as 68198-1-PBS)

    WB analysis using 68198-1-Ig (same clone as 68198-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68198-1-Ig (CYP2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68198-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50889-4

    Cytometric bead array standard curve of MP50889-4

    Cytometric bead array standard curve of MP50889-4, CYP2S1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68198-1-PBS. Detection antibody: 68198-6-PBS. Standard:Ag4605. Range: 0.391-100 ng/mL.

CYP2S1 Monoclonal antibody, PBS Only (Capture)

CYP2S1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 68198-1-PBS

产品说明书

CloneNo. 3E5D4

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, Cytometric bead array, Indirect ELISA

别名

EC:5.3.99.5, EC:4.2.1.152, EC:1.14.14.-, Cytochrome P450 2S1, CYPIIS1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68198-1-PBS targets CYP2S1 as part of a matched antibody pair:

MP50889-4: 68198-1-PBS capture and 68198-6-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 CYP2S1 fusion protein Ag4605 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cytochrome P450, family 2, subfamily S, polypeptide 1
别名 EC:5.3.99.5, EC:4.2.1.152, EC:1.14.14.-, Cytochrome P450 2S1, CYPIIS1
计算分子量 504 aa, 56 kDa
观测分子量50-56 kDa
GenBank蛋白编号BC033691
基因名称 CYP2S1
Gene ID (NCBI) 29785
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96SQ9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cytochrome P450 (CYP) family of isoenzymes are involved in the metabolism of diverse endogenous compounds and detoxification of exogenous agents. Cytochrome P450 2S1 (CYP2S1) is one 'orphan' CYP, and is particularly expressed in epithe-lial cells of tissues which are exposed to the environment such as the skin, respiratory, gastrointes-tinal, and urinary tracts. CYP2S1 is associated primarily with the synthesis and metabo-lism of lipids including prostaglandins (PGE2) and retinoids. In addition, CYP2S1 expression is associated with patient survival in breast cancer.