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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CYP2W1 Monoclonal antibody

CYP2W1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B10H2

Cat No : CL488-67419

Print datasheet

Synonyms

EC:1.14.14.-, Cytochrome P450 2W1, CYPIIW1, 2B10H2

验证数据展示

  • IF Staining of HepG2 using CL488-67419

    IF Staining of HepG2 using CL488-67419

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CYP2W1 antibody (CL488-67419, Clone: 2B10H2 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HUVEC using CL488-67419

    FC experiment of HUVEC using CL488-67419

    1x10^6 HUVEC cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CYP2W1 Monoclonal antibody (CL488-67419, Clone:2B10H2)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHUVEC cells
For other applications, we recommend the unconjugated version of this antibody, 67419-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67419 targets CYP2W1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CYP2W1 fusion protein Ag29982 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytochrome P450, family 2, subfamily W, polypeptide 1
Synonyms EC:1.14.14.-, Cytochrome P450 2W1, CYPIIW1, 2B10H2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 45-54 kDa
GenBank Accession NumberBC025761
Gene Symbol CYP2W1
Gene ID (NCBI) 54905
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TAV3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome P450s have been proposed to be of importance both in carcinogenesis, by activating precarcinogens, and as determinants of cancer chemotherapy, where they participate in activation or inactivation of anti-cancer drugs. CYP2W1 is exclusively expressed in transformed tissue in the adult human, mainly in colon tumors. It is a potential drug target in cancer therapy. CYP2W1 has a molecular weight of 52-54 kDa, and 30/45 kDa of potential isoforms.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CYP2W1 antibody CL488-67419Download protocol
Standard Protocols
Click here to view our Standard Protocols