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验证数据展示

  • IF Staining of HepG2 using CL488-67419

    IF Staining of HepG2 using CL488-67419

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CYP2W1 antibody (CL488-67419, Clone: 2B10H2 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HUVEC using CL488-67419

    FC experiment of HUVEC using CL488-67419

    1x10^6 HUVEC cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CYP2W1 Monoclonal antibody (CL488-67419, Clone:2B10H2)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CYP2W1 Monoclonal antibody

CYP2W1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67419

产品说明书

CloneNo. 2B10H2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

EC:1.14.14.-, Cytochrome P450 2W1, CYPIIW1, 2B10H2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHUVEC cells
For other applications, we recommend the unconjugated version of this antibody, 67419-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67419 targets CYP2W1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CYP2W1 fusion protein Ag29982 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cytochrome P450, family 2, subfamily W, polypeptide 1
别名 EC:1.14.14.-, Cytochrome P450 2W1, CYPIIW1, 2B10H2
计算分子量 54 kDa
观测分子量 45-54 kDa
GenBank蛋白编号BC025761
基因名称 CYP2W1
Gene ID (NCBI) 54905
RRIDAB_3672947
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8TAV3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome P450s have been proposed to be of importance both in carcinogenesis, by activating precarcinogens, and as determinants of cancer chemotherapy, where they participate in activation or inactivation of anti-cancer drugs. CYP2W1 is exclusively expressed in transformed tissue in the adult human, mainly in colon tumors. It is a potential drug target in cancer therapy. CYP2W1 has a molecular weight of 52-54 kDa, and 30/45 kDa of potential isoforms.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CYP2W1 antibody CL488-67419Download protocol
Standard Protocols
Click here to view our Standard Protocols