验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, pig liver tissue, pig gall bladder tissue, mouse lung tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue, rat liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CYP3A4 Ready-To-Use IHC Kit. CYP3A4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 3 publications below |
产品信息
67110-1-Ig targets CYP3A4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 | CYP3A4 fusion protein Ag13074 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 3, subfamily A, polypeptide 4 |
| 别名 | CYP3A3, CYP3A, CP34, CP33, Cholesterol 25-hydroxylase |
| 计算分子量 | 503 aa, 57 kDa |
| 观测分子量 | 51-57 kDa |
| GenBank蛋白编号 | BC101631 |
| 基因名称 | CYP3A4 |
| Gene ID (NCBI) | 1576 |
| RRID | AB_2882414 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08684 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP3A4 antibody 67110-1-Ig | Download protocol |
| IHC protocol for CYP3A4 antibody 67110-1-Ig | Download protocol |
| IF protocol for CYP3A4 antibody 67110-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Rep Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells. | ||
Methods Mol Biol Homogeneous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells. | ||
Acta Biomater Hepar-on-a-sensor-platform with hybridization chain reaction amplification strategy to intuitively monitor the hepatoxicity of natural compounds |
