CYP3A4 Monoclonal antibody
CYP3A4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF/ICC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2F11C4
验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, pig liver tissue, pig gall bladder tissue, mouse lung tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue, rat liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CYP3A4 Ready-To-Use IHC Kit. CYP3A4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 3 publications below |
产品信息
67110-1-Ig targets CYP3A4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Immunogen | CYP3A4 fusion protein Ag13074 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cytochrome P450, family 3, subfamily A, polypeptide 4 |
| Synonyms | CYP3A3, CYP3A, CP34, CP33, Cholesterol 25-hydroxylase |
| Calculated Molecular Weight | 503 aa, 57 kDa |
| Observed Molecular Weight | 51-57 kDa |
| GenBank Accession Number | BC101631 |
| Gene Symbol | CYP3A4 |
| Gene ID (NCBI) | 1576 |
| RRID | AB_2882414 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08684 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP3A4 antibody 67110-1-Ig | Download protocol |
| IHC protocol for CYP3A4 antibody 67110-1-Ig | Download protocol |
| IF protocol for CYP3A4 antibody 67110-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Rep Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells. | ||
Methods Mol Biol Homogeneous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells. | ||
Acta Biomater Hepar-on-a-sensor-platform with hybridization chain reaction amplification strategy to intuitively monitor the hepatoxicity of natural compounds |
