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CYP3A4 Monoclonal antibody, PBS Only (Detector)

CYP3A4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F11C4

Cat no : 67110-1-PBS

Print datasheet

Synonyms

CYP3A3, CYP3A, CP34, CP33, Cholesterol 25-hydroxylase

验证数据展示

  • IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    WB analysis of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP3A4 antibody included
  • IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP3A4 antibody included
  • IF Staining of rat liver using 67110-1-Ig (same clone as 67110-1-PBS)

    IF Staining of rat liver using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67110-1-Ig (same clone as 67110-1-PBS)

    IF Staining of HepG2 using 67110-1-Ig (same clone as 67110-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50718-4

    Cytometric bead array standard curve of MP50718-4

    Cytometric bead array standard curve of MP50718-4, CYP3A4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67110-6-PBS. Detection antibody: 67110-1-PBS. Standard:Ag13074. Range: 0.781-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy CYP3A4 Ready-To-Use IHC Kit. CYP3A4 primary antibody included.

产品信息

67110-1-PBS targets CYP3A4 as part of a matched antibody pair:

MP50718-4: 67110-6-PBS capture and 67110-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CYP3A4 fusion protein Ag13074 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytochrome P450, family 3, subfamily A, polypeptide 4
Synonyms CYP3A3, CYP3A, CP34, CP33, Cholesterol 25-hydroxylase
Calculated Molecular Weight 503 aa, 57 kDa
Observed Molecular Weight 51-57 kDa
GenBank Accession NumberBC101631
Gene Symbol CYP3A4
Gene ID (NCBI) 1576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08684
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C