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验证数据展示

  • WB analysis using 68576-1-Ig

    WB analysis using 68576-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68576-1-Ig (CYP46A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68576-1-Ig

    IHC staining of mouse brain using 68576-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68576-1-Ig (CYP46A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68576-1-Ig

    IHC staining of mouse brain using 68576-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68576-1-Ig (CYP46A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CYP46A1 Monoclonal antibody

CYP46A1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68576-1-Ig

产品说明书

CloneNo. 1E10D3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Rabbit, Pig

应用

WB, IHC, ELISA

别名

CH24H, Cholesterol 24 hydroxylase, CP46, CYP46, CYP46A1, Cytochrome P450 46A1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68576-1-Ig targets CYP46A1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Rabbit, Pig
免疫原 CYP46A1 fusion protein Ag16786 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cytochrome P450, family 46, subfamily A, polypeptide 1
别名 CH24H, Cholesterol 24 hydroxylase, CP46, CYP46, CYP46A1, Cytochrome P450 46A1
计算分子量 500 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC022539
基因名称 CYP46A1
Gene ID (NCBI) 10858
RRIDAB_3085276
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6A2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome P450 46A1 (CYP46A1), also named as CYP46, P450 46A1, CH24H and Cholesterol 24-hydroxylase, belongs to the cytochrome P450 family. CYP46A1 is a central nervous system-specific enzyme, and can freely cross the blood-brain barrier and be degraded in the liver. It is involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. CYP46A1 is significantly upregulated in hepatic lymphomyeloid cells compared with ESCs.(PMID:20039312)

实验方案

Product Specific Protocols
WB protocol for CYP46A1 antibody 68576-1-IgDownload protocol
IHC protocol for CYP46A1 antibody 68576-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols