验证数据展示
经过测试的应用
Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
20012-1-AP targets CYP4F11-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | IHC, IF/ICC, ELISA Application Description |
文献引用应用 | IHC |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cytochrome P450, family 4, subfamily F, polypeptide 11 |
别名 | CYP4F11, CYP4F11-Specific, CYPIVF11, Cytochrome P450 4F11 |
计算分子量 | 60 kDa |
观测分子量 | 60 kDa |
GenBank蛋白编号 | NM_001128932 |
基因名称 | CYP4F11 |
Gene ID (NCBI) | 57834 |
RRID | AB_10665946 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9HBI6 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CYP4F11 belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F11 catalyze the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP4F11. It has no cross reaction to other CYP4F member.This antibody is specific to CYP4F11.
实验方案
Product Specific Protocols | |
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IHC protocol for CYP4F11-Specific antibody 20012-1-AP | Download protocol |
IF protocol for CYP4F11-Specific antibody 20012-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int Immunopharmacol Regulation of cytochrome P450 4F11 expression by liver X receptor alpha. | ||
Biochem Biophys Rep Targeting estrogen mediated CYP4F2/CYP4F11-20-HETE metabolic disorder decelerates tumorigenesis in ER+ breast cancer |