验证数据展示
经过测试的应用
| Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
20012-1-AP targets CYP4F11-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, IF/ICC, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cytochrome P450, family 4, subfamily F, polypeptide 11 |
| Synonyms | CYP4F11, CYP4F11-Specific, CYPIVF11, Cytochrome P450 4F11 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | NM_001128932 |
| Gene Symbol | CYP4F11 |
| Gene ID (NCBI) | 57834 |
| RRID | AB_10665946 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBI6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CYP4F11 belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F11 catalyze the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP4F11. It has no cross reaction to other CYP4F member.This antibody is specific to CYP4F11.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CYP4F11-Specific antibody 20012-1-AP | Download protocol |
| IF protocol for CYP4F11-Specific antibody 20012-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Regulation of cytochrome P450 4F11 expression by liver X receptor alpha. | ||
Biochem Biophys Rep Targeting estrogen mediated CYP4F2/CYP4F11-20-HETE metabolic disorder decelerates tumorigenesis in ER+ breast cancer |