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CYP4F11-Specific Polyclonal antibody

CYP4F11-Specific Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20012-1-AP

Print datasheet

Synonyms

CYP4F11, CYP4F11-Specific, CYPIVF11, Cytochrome P450 4F11

验证数据展示

  • IHC staining of human hepatocirrhosis using 20012-1-AP

    IHC staining of human hepatocirrhosis using 20012-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20012-1-AP (CYP4F11-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 20012-1-AP

    IHC staining of human hepatocirrhosis using 20012-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20012-1-AP (CYP4F11-Specific antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20012-1-AP

    IF Staining of HepG2 using 20012-1-AP

    Immunofluorescent analysis of HepG2 cells, using CYP4F11 antibody 20012-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

20012-1-AP targets CYP4F11-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 4, subfamily F, polypeptide 11
Synonyms CYP4F11, CYP4F11-Specific, CYPIVF11, Cytochrome P450 4F11
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberNM_001128932
Gene Symbol CYP4F11
Gene ID (NCBI) 57834
RRIDAB_10665946
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP4F11 belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F11 catalyze the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP4F11. It has no cross reaction to other CYP4F member.This antibody is specific to CYP4F11.

实验方案

Product Specific Protocols
IHC protocol for CYP4F11-Specific antibody 20012-1-APDownload protocol
IF protocol for CYP4F11-Specific antibody 20012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouse

Int Immunopharmacol

Regulation of cytochrome P450 4F11 expression by liver X receptor alpha.

Authors - Tao Zhang
View Article
human,mouseIHC

Biochem Biophys Rep

Targeting estrogen mediated CYP4F2/CYP4F11-20-HETE metabolic disorder decelerates tumorigenesis in ER+ breast cancer

Authors - Juan Yang
View Article