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CoraLite® Plus 488-conjugated Calbindin-D28k Monoclonal antibody

Calbindin-D28k Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F8B9

Cat no : CL488-66394

Print datasheet

Synonyms

Calbindin, CALB1, Calbindin D28, calbindin 1, 28kDa, CALB

验证数据展示

  • IF Staining of mouse cerebellum using CL488-66394

    IF Staining of mouse cerebellum using CL488-66394

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using CL488-66394

    IF Staining of mouse cerebellum using CL488-66394

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using CL488-66394

    IF Staining of mouse cerebellum using CL488-66394

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inmouse cerebellum tissue, rat cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy Calbindin-D28k Ready-To-Use IHC Kit. Calbindin-D28k primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66394-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66394 targets Calbindin-D28k in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Calbindin-D28k fusion protein Ag5861 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calbindin 1, 28kDa
Synonyms Calbindin, CALB1, Calbindin D28, calbindin 1, 28kDa, CALB
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC006478
Gene Symbol Calbindin
Gene ID (NCBI) 793
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05937
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Calbindin-D28k antibody CL488-66394Download protocol
Standard Protocols
Click here to view our Standard Protocols