验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
Positive IHC detected in | rat brain tissue, mouse brain tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28270-1-AP targets Calmodulin 1/2/3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | Calmodulin 1/2/3 fusion protein Ag28000 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | calmodulin 2 (phosphorylase kinase, delta) |
别名 | CALM, CALM1, CALM2, CALM3, CALML2, Calmodulin, Calmodulin 1/2/3, CaM, CAM1, CAM2, CAM3, CAMB, CAMC, CAMII, CAMIII, PHKD, PHKD2 |
计算分子量 | 17 kDa |
观测分子量 | 17-22 kDa |
GenBank蛋白编号 | BC006464 |
基因名称 | Calmodulin 1 |
Gene ID (NCBI) | 805 |
RRID | AB_3669650 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P62158 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calmodulin is the most widely distributed and most versatile member of a family of calcium-binding proteins which probably serve as receptors for the Ca2+ signal. Through the regulation of a wide variety of intracellular enzymes, calmodulin plays a key role in many physiological processes (PMID: 6313166).
实验方案
Product Specific Protocols | |
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WB protocol for Calmodulin 1/2/3 antibody 28270-1-AP | Download protocol |
IHC protocol for Calmodulin 1/2/3 antibody 28270-1-AP | Download protocol |
IF protocol for Calmodulin 1/2/3 antibody 28270-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |