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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis of mouse skeletal muscle using 10415-1-AP (same clone as 10415-1-PBS)

    WB analysis of mouse skeletal muscle using 10415-1-AP (same clone as 10415-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10415-1-AP (Calpain 3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 10415-1-AP (same clone as 10415-1-PBS)

    IHC staining of human urothelial carcinoma using 10415-1-AP (same clone as 10415-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10415-1-AP (Calpain 3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN3 antibody included
  • IHC staining of human urothelial carcinoma using 10415-1-AP (same clone as 10415-1-PBS)

    IHC staining of human urothelial carcinoma using 10415-1-AP (same clone as 10415-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10415-1-AP (Calpain 3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN3 antibody included

Calpain 3 Polyclonal antibody, PBS Only

Calpain 3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10415-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CAPN3 Ready-To-Use IHC Kit. CAPN3 primary antibody included.

产品信息

10415-1-PBS targets Calpain 3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Calpain 3 fusion protein Ag0671 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calpain 3, (p94)
别名
计算分子量 94 kDa
观测分子量 90-105 kDa, 55-60 kDa, 30-35 kDa
GenBank蛋白编号BC003521
基因名称 CAPN3
Gene ID (NCBI) 825
RRIDAB_2069647
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20807
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).