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Calponin Polyclonal antibody, PBS Only

Calponin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24855-1-PBS

Print datasheet

Synonyms

CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin

验证数据展示

  • WB analysis of mouse stomach using 24855-1-AP (same clone as 24855-1-PBS)

    WB analysis of mouse stomach using 24855-1-AP (same clone as 24855-1-PBS)

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24855-1-AP (Calponin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 24855-1-AP (same clone as 24855-1-PBS)

    IHC staining of human colon using 24855-1-AP (same clone as 24855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24855-1-AP (Calponin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 24855-1-AP (same clone as 24855-1-PBS)

    IHC staining of human heart using 24855-1-AP (same clone as 24855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 24855-1-AP (same clone as 24855-1-PBS)

    IHC staining of human heart using 24855-1-AP (same clone as 24855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 24855-1-AP (same clone as 24855-1-PBS)

    IF Staining of mouse heart using 24855-1-AP (same clone as 24855-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calponin antibody (24855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 24855-1-AP (same clone as 24855-1-PBS)

    IF Staining of C2C12 using 24855-1-AP (same clone as 24855-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (24855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

产品信息

24855-1-PBS targets Calponin in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Calponin fusion protein Ag21384 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calponin 1, basic, smooth muscle
Synonyms CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC022015
Gene Symbol Calponin
Gene ID (NCBI) 1264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51911
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kDa protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.