验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66419 targets Calsequestrin 2 in IF-P applications and shows reactivity with human, rat, pig, mouse samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, rat, pig, mouse |
| 免疫原 | Calsequestrin 2 fusion protein Ag13246 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | calsequestrin 2 (cardiac muscle) |
| 别名 | Calsequestrin 2, CASQ2, FLJ26321, FLJ93514, PDIB2 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC022288 |
| 基因名称 | Calsequestrin 2 |
| Gene ID (NCBI) | 845 |
| RRID | AB_2919322 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O14958 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Calsequestrin 2 antibody CL488-66419 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |