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验证数据展示

  • IF Staining of mouse brain using CL488-17978

    IF Staining of mouse brain using CL488-17978

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 Cannabinoid receptor 1 antibody (CL488-17978) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated Cannabinoid receptor 1 Polyclonal antibody

Cannabinoid receptor 1 Polyclonal Antibody for IF-P
Cat No. CL488-17978

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

CANN6, Cannabinoid receptor 1, cannabinoid receptor 1 (brain), CB R, CB1, CB1A, CB1K5, CB1R, CNR, CNR1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 17978-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17978 targets Cannabinoid receptor 1 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Cannabinoid receptor 1 fusion protein Ag12371 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cannabinoid receptor 1 (brain)
Synonyms CANN6, Cannabinoid receptor 1, cannabinoid receptor 1 (brain), CB R, CB1, CB1A, CB1K5, CB1R, CNR, CNR1
Calculated Molecular Weight 472 aa, 53 kDa
Observed Molecular Weight45 kDa, 66 kDa
GenBank Accession NumberBC074812
Gene Symbol Cannabinoid receptor 1
Gene ID (NCBI) 1268
RRIDAB_3084014
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21554
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cannabinoid receptor 1 (CNR1, or CB1) and CNR2 (CB2) are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. The CB1 receptor is expressed mainly in the brain. The CB2 receptor is expressed mainly in the immune system and in hematopoietic cells. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cannabinoid receptor 1 antibody CL488-17978Download protocol
Standard Protocols
Click here to view our Standard Protocols