Cardiac Troponin I Monoclonal antibody

Cardiac Troponin I Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1D5D6

Cat no : 66376-1-Ig

Print datasheet

Synonyms

cTnI, TNNI3, 1D5D6



经过测试的应用

Positive WB detected inhuman heart tissue, rat heart tissue, mouse heart tissue, pig heart tissue, rabbit heart tissue
Positive IHC detected inhuman heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy TNNI3 Ready-To-Use IHC Kit. TNNI3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66376-1-Ig targets Cardiac Troponin I in WB, IHC, IF-P, ELISA, ISFET applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IF, ISFET
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig
Immunogen Cardiac Troponin I fusion protein Ag16758 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name troponin I type 3 (cardiac)
Synonyms cTnI, TNNI3, 1D5D6
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC096165
Gene Symbol Cardiac Troponin I
Gene ID (NCBI) 7137
RRIDAB_2881753
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19429
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.

实验方案

Product Specific Protocols
WB protocol for Cardiac Troponin I antibody 66376-1-IgDownload protocol
IHC protocol for Cardiac Troponin I antibody 66376-1-IgDownload protocol
IF protocol for Cardiac Troponin I antibody 66376-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

ACS Nano

Collagen-Targeting Self-Assembled Nanoprobes for Multimodal Molecular Imaging and Quantification of Myocardial Fibrosis in a Rat Model of Myocardial Infarction

Authors - Fang Li
humanISFET

Biosens Bioelectron

A handheld testing device for the fast and ultrasensitive recognition of cardiac troponin I via an ion-sensitive field-effect transistor

Authors - Yiqing Wang
pigWB

Stem Cell Reports

Physiological calcium combined with electrical pacing accelerates maturation of human engineered heart tissue

Authors - Shi Shen
humanIF

Stem Cell Res Ther

Intravenously transplanted mesenchymal stromal cells: a new endocrine reservoir for cardioprotection.

Authors - Anan Huang
mouseIF

J Am Heart Assoc

Cardiomyocyte-Specific Snrk Prevents Inflammation in the Heart.

Authors - Karthikeyan Thirugnanam
mouseWB

J Cell Mol Med

Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress.

Authors - Linmao Lyu