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验证数据展示

  • WB analysis using 66376-1-Ig (same clone as 66376-1-PBS)

    WB analysis using 66376-1-Ig (same clone as 66376-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (66376-1-Ig, Clone: 1D5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNNI3 antibody included
  • IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNNI3 antibody included
  • IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNNI3 antibody included
  • WB analysis of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    WB analysis of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Human heart tissue and skeletal muscle tissue (negative control) were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:20,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    WB analysis of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Mouse heart tissue (6 ug/lane) was subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed mouse heart tissue using 66376-1-Ig(Cardiac Troponin I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP51198-1

    Sandwich ELISA standard curve of MP51198-1

    Sandwich ELISA standard curve of MP51198-1, Human Cardiac Troponin I Monoclonal Matched Antibody Pair - PBS only. 66376-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16758. 66376-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP51198-1

    Sample test of MP51198-1

    The mean Cardiac Troponin I concentration was determined to be 220.78 ng/mL in mouse heart cell extract based on a 7.10 mg/mL extract load.

  • Sample test of MP51198-1

    Sample test of MP51198-1

    Cytometric bead array in cell lysate using MP51198-1, Cardiac Troponin I Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66376-2-PBS. Detection antibody: 66376-1-PBS. Cell lysate: Rabbit muscle, Rabbit heart.

  • Cytometric bead array standard curve of MP51198-1

    Cytometric bead array standard curve of MP51198-1

    Cytometric bead array standard curve of MP51198-1, Cardiac Troponin I Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66376-2-PBS. Detection antibody: 66376-1-PBS. Standard:Ag16758. Range: 12.5-400 ng/mL.

Cardiac Troponin I Monoclonal antibody, PBS Only (Detector)

Cardiac Troponin I Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 66376-1-PBS

产品说明书

CloneNo. 1D5D6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

cTnI, 1D5D6, TNNI3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TNNI3 Ready-To-Use IHC Kit. TNNI3 primary antibody included.

产品信息

66376-1-PBS targets Cardiac Troponin I as part of a matched antibody pair:

MP51198-1: 66376-2-PBS capture and 66376-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Cardiac Troponin I fusion protein Ag16758 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 troponin I type 3 (cardiac)
别名 cTnI, 1D5D6, TNNI3
计算分子量 210 aa, 24 kDa
观测分子量 26-28 kDa
GenBank蛋白编号BC096165
基因名称 Cardiac Troponin I
Gene ID (NCBI) 7137
RRIDAB_2881753
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP19429
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.