验证数据展示
经过测试的应用
Positive WB detected in | Raji cells, THP-1 cells, U-937 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81482-1-RR targets Caspase 1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse, rat |
免疫原 | Caspase 1 fusion protein Ag11143 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) |
别名 | CASP1, Caspase1, Caspase-1, CASP-1, CASP 1 |
计算分子量 | 404 aa, 45 kDa |
观测分子量 | 30 kDa, 35 kDa, 43-45 kDa |
GenBank蛋白编号 | BC062327 |
基因名称 | Caspase 1 |
Gene ID (NCBI) | 834 |
RRID | AB_2935555 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P29466 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the splicing isoforms of caspase 1.
实验方案
Product Specific Protocols | |
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WB protocol for Caspase 1 antibody 81482-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Scutellarin Rescued Mitochondrial Damage through Ameliorating Mitochondrial Glucose Oxidation via the Pdk-Pdc Axis | ||
Phytomedicine Systemic pharmacology reveal the mechanism by which the Qiangjin Zhuanggu Qufeng mixture inhibits LPS-induced pyroptosis of rat nucleus pulposus cells | ||
Int Immunopharmacol Triggering receptor expressed on myeloid cells-1 aggravates obliterative bronchiolitis via enhancing the proinflammatory phenotype of macrophages | ||
Antioxidants (Basel) Bisdemethoxycurcumin Alleviates Dextran Sodium Sulfate-Induced Colitis via Inhibiting NLRP3 Inflammasome Activation and Modulating the Gut Microbiota in Mice | ||
PLoS One Forsythoside B alleviates cerebral ischemia-reperfusion injury via inhibiting NLRP3 inflammasome mediated by SIRT1 activation | ||
Biochem Biophys Res Commun Uric acid-lowering effect of harpagoside and its protective effect against hyperuricemia-induced renal injury in mice |