Caspase 1 Recombinant antibody

Caspase 1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1O18

Cat no : 81482-1-RR

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Synonyms

CASP1, Caspase1, Caspase-1, CASP-1, CASP 1



经过测试的应用

Positive WB detected inRaji cells, THP-1 cells, U-937 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81482-1-RR targets Caspase 1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen Caspase 1 fusion protein Ag11143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
Synonyms CASP1, Caspase1, Caspase-1, CASP-1, CASP 1
Calculated Molecular Weight 404 aa, 45 kDa
Observed Molecular Weight30 kDa, 35 kDa, 43-45 kDa
GenBank Accession NumberBC062327
Gene Symbol Caspase 1
Gene ID (NCBI) 834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29466
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the splicing isoforms of caspase 1.

实验方案

Product Specific Protocols
WB protocol for Caspase 1 antibody 81482-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Scutellarin Rescued Mitochondrial Damage through Ameliorating Mitochondrial Glucose Oxidation via the Pdk-Pdc Axis

Authors - Ning Sheng
ratWB,IF

Phytomedicine

Systemic pharmacology reveal the mechanism by which the Qiangjin Zhuanggu Qufeng mixture inhibits LPS-induced pyroptosis of rat nucleus pulposus cells

Authors - Dong Wang
mouseWB

Antioxidants (Basel)

Bisdemethoxycurcumin Alleviates Dextran Sodium Sulfate-Induced Colitis via Inhibiting NLRP3 Inflammasome Activation and Modulating the Gut Microbiota in Mice

Authors - Jingfei Zhang
ratWB

PLoS One

Forsythoside B alleviates cerebral ischemia-reperfusion injury via inhibiting NLRP3 inflammasome mediated by SIRT1 activation

Authors - Qiaoyu Li
mouseWB

Brain Res

Neuronal pyroptosis mediated by STAT3 in early brain injury after subarachnoid hemorrhage

Authors - Shengjie Tang
mouse,ratWB

Heliyon

Asprosin promotes vascular inflammation via TLR4-NFκB-mediated NLRP3 inflammasome activation in hypertension

Authors - Rui Ge