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CoraLite® Plus 750-conjugated Caspase 1 Recombinant antibody

Caspase 1 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1O18

Cat No : CL750-81482

Print datasheet

Synonyms

CASP1, Caspase1, Caspase-1, CASP-1, CASP 1

验证数据展示

  • WB analysis using CL750-81482

    WB analysis using CL750-81482

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81482 (Caspase 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inTHP-1 cells, U-937 cells
For other applications, we recommend the unconjugated version of this antibody, 81482-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-81482 targets Caspase 1 in WB applications and shows reactivity with human samples.

Tested Applications WB Application Description
Tested Reactivity human
Immunogen Caspase 1 fusion protein Ag11143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
Synonyms CASP1, Caspase1, Caspase-1, CASP-1, CASP 1
Calculated Molecular Weight 404 aa, 45 kDa
Observed Molecular Weight30 kDa, 35 kDa, 43-45 kDa
GenBank Accession NumberBC062327
Gene Symbol Caspase 1
Gene ID (NCBI) 834
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29466
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the splicing isoforms of caspase 1.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Caspase 1 antibody CL750-81482Download protocol
Standard Protocols
Click here to view our Standard Protocols