验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, U-937 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL750-81482 targets Caspase 1 in WB applications and shows reactivity with human samples.
经测试应用 | WB Application Description |
经测试反应性 | human |
免疫原 | Caspase 1 fusion protein Ag11143 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) |
别名 | CASP1, Caspase1, Caspase-1, CASP-1, CASP 1 |
计算分子量 | 404 aa, 45 kDa |
观测分子量 | 30 kDa, 35 kDa, 43-45 kDa |
GenBank蛋白编号 | BC062327 |
基因名称 | Caspase 1 |
Gene ID (NCBI) | 834 |
RRID | AB_3673728 |
偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
最大激发/发射波长 | 755 nm / 780 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P29466 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the splicing isoforms of caspase 1.
实验方案
Product Specific Protocols | |
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WB protocol for CL Plus 750 Caspase 1 antibody CL750-81482 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |