验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, U-937 cells |
For other applications, we recommend the unconjugated version of this antibody, 81482-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81482 targets Caspase 1 in WB applications and shows reactivity with human samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | human |
| Immunogen | Caspase 1 fusion protein Ag11143 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) |
| Synonyms | CASP1, Caspase1, Caspase-1, CASP-1, CASP 1 |
| Calculated Molecular Weight | 404 aa, 45 kDa |
| Observed Molecular Weight | 30 kDa, 35 kDa, 43-45 kDa |
| GenBank Accession Number | BC062327 |
| Gene Symbol | Caspase 1 |
| Gene ID (NCBI) | 834 |
| RRID | AB_3673728 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P29466 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the splicing isoforms of caspase 1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 Caspase 1 antibody CL750-81482 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |