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验证数据展示

  • WB analysis using 84735-1-RR

    WB analysis using 84735-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84735-1-RR (Caspase 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 84735-1-RR

    FC experiment of Jurkat using 84735-1-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Caspase 1 Recombinant antibody (84735-1-RR, Clone:241967A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Caspase 1 Recombinant antibody

Caspase 1 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84735-1-RR

产品说明书

CloneNo. 241967A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

CASP1, Caspase1, Caspase-1, CASP-1, CASP 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, U-937 cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84735-1-RR targets Caspase 1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
别名 CASP1, Caspase1, Caspase-1, CASP-1, CASP 1
计算分子量 404 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC062327
基因名称 Caspase 1
Gene ID (NCBI) 834
RRIDAB_3672119
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP29466
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the splicing isoforms of caspase 1.

实验方案

Product Specific Protocols
WB protocol for Caspase 1 antibody 84735-1-RRDownload protocol
FC protocol for Caspase 1 antibody 84735-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols