验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84752-1-RR targets Caspase 1 in IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) |
| 别名 | CASP1, Caspase1, Caspase-1, CASP-1, CASP 1 |
| 计算分子量 | 404 aa, 45 kDa |
| GenBank蛋白编号 | BC062327 |
| 基因名称 | Caspase 1 |
| Gene ID (NCBI) | 834 |
| RRID | AB_3672135 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P29466 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Caspase 1 antibody 84752-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |