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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 85196-1-RR (same clone as 85196-1-PBS)

    WB analysis using 85196-1-RR (same clone as 85196-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85196-1-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85196-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 85196-1-RR (same clone as 85196-1-PBS)

    FC experiment of HepG2 using 85196-1-RR (same clone as 85196-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Caspase 10 Recombinant Antibody (85196-1-RR, Clone:242353A8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85196-1-PBS in a different storage buffer formulation.

  • FC experiment of K562 using 85196-1-RR (same clone as 85196-1-PBS)

    FC experiment of K562 using 85196-1-RR (same clone as 85196-1-PBS)

    1x10^6 K562 were intracellularly stained with 0.25 ug Caspase 10 Recombinant Antibody (85196-1-RR, Clone:242353A8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85196-1-PBS in a different storage buffer formulation.

Caspase 10 Recombinant antibody, PBS Only

Caspase 10 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 85196-1-PBS

产品说明书

CloneNo. 242353A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

CASP10, Caspase10, CASP-10, CASP 10, Apoptotic protease Mch-4

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85196-1-PBS targets Caspase 10 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 caspase 10, apoptosis-related cysteine peptidase
别名 CASP10, Caspase10, CASP-10, CASP 10, Apoptotic protease Mch-4
计算分子量 59 kDa
观测分子量59 kDa
GenBank蛋白编号BC042844
基因名称 Caspase 10
Gene ID (NCBI) 843
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ92851
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CASP10(Caspase-10) is also named as MCH4 and belongs to the peptidase C14A family. It is recruited to the CD99 and p55 tumor necrosis factor receptor signaling complexes and promoting CD95 (APO1/Fas) apoptosis. It may also play a critical role in PTK7-knockdown-induced apoptosis, downstream of mitochondria(PMID:21103379). This protein can be Cleavage by granzyme B and autocatalytic activity generate two active subunits. Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A)(PMID:16446975), familial non-Hodgkin lymphoma (NHL)(PMID:12010812) and gastric cancer (GASC)(PMID:11973654). The full length has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing.