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验证数据展示

  • WB analysis using 85196-1-RR

    WB analysis using 85196-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85196-1-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 85196-1-RR

    FC experiment of HepG2 using 85196-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Caspase 10 Recombinant Antibody (85196-1-RR, Clone:242353A8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of K562 using 85196-1-RR

    FC experiment of K562 using 85196-1-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug Caspase 10 Recombinant Antibody (85196-1-RR, Clone:242353A8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Caspase 10 Recombinant antibody

Caspase 10 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 85196-1-RR

产品说明书

CloneNo. 242353A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

CASP10, Caspase10, CASP-10, CASP 10, Apoptotic protease Mch-4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A431 cells, K-562 cells
Positive FC (Intra) detected inHepG2 cells, K562

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85196-1-RR targets Caspase 10 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 caspase 10, apoptosis-related cysteine peptidase
别名 CASP10, Caspase10, CASP-10, CASP 10, Apoptotic protease Mch-4
计算分子量 59 kDa
观测分子量59 kDa
GenBank蛋白编号BC042844
基因名称 Caspase 10
Gene ID (NCBI) 843
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ92851
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CASP10(Caspase-10) is also named as MCH4 and belongs to the peptidase C14A family. It is recruited to the CD99 and p55 tumor necrosis factor receptor signaling complexes and promoting CD95 (APO1/Fas) apoptosis. It may also play a critical role in PTK7-knockdown-induced apoptosis, downstream of mitochondria(PMID:21103379). This protein can be Cleavage by granzyme B and autocatalytic activity generate two active subunits. Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A)(PMID:16446975), familial non-Hodgkin lymphoma (NHL)(PMID:12010812) and gastric cancer (GASC)(PMID:11973654). The full length has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for Caspase 10 antibody 85196-1-RRDownload protocol
FC protocol for Caspase 10 antibody 85196-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols