• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 66517-1-Ig (same clone as 66517-1-PBS)

    WB analysis of Jurkat using 66517-1-Ig (same clone as 66517-1-PBS)

    Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66517-1-Ig (Caspase 2/P32/P18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66517-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50435-1

    Cytometric bead array standard curve of MP50435-1

    Cytometric bead array standard curve of MP50435-1, Caspase 2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66517-2-PBS. Detection antibody: 66517-1-PBS. Standard:Ag20141. Range: 0.098-25 ng/mL.

Caspase 2/P32/P18 Monoclonal antibody, PBS Only (Detector)

Caspase 2/P32/P18 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 66517-1-PBS

产品说明书

CloneNo. 2G4C2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

CASP2, Caspase 2, 2G4C2, CASP 2, CASP-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy Caspase 2 Ready-To-Use IHC Kit. Caspase 2 primary antibody included.

产品信息

66517-1-PBS targets Caspase 2/P32/P18 as part of a matched antibody pair:

MP50435-1: 66517-2-PBS capture and 66517-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Caspase 2/P32/P18 fusion protein Ag20141 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase 2, apoptosis-related cysteine peptidase
别名 CASP2, Caspase 2, 2G4C2, CASP 2, CASP-2
计算分子量 452 aa, 51 kDa
观测分子量48-51 kDa, 32 kDa, 18 kDa
GenBank蛋白编号BC002427
基因名称 Caspase 2
Gene ID (NCBI) 835
RRIDAB_2881880
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP42575
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).This antibody can recognize the 32 kDa pro-caspase 2 as well as 18 kDa cleaved-caspase 2.