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验证数据展示

  • WB analysis of Jurkat using 66517-1-Ig

    WB analysis of Jurkat using 66517-1-Ig

    Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66517-1-Ig (Caspase 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Caspase 2/p32/p18 Monoclonal antibody

Caspase 2/p32/p18 Monoclonal Antibody for WB, ELISA
Cat No. 66517-1-Ig

Print datasheet

CloneNo. 2G4C2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

CASP 2, CASP2, Caspase 2, Caspase 2/p32/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy Caspase 2 Ready-To-Use IHC Kit. Caspase 2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66517-1-Ig targets Caspase 2/p32/p18 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Caspase 2/p32/p18 fusion protein Ag20141 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caspase 2, apoptosis-related cysteine peptidase
Synonyms CASP 2, CASP2, Caspase 2, Caspase 2/p32/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1
Calculated Molecular Weight 452 aa, 51 kDa
Observed Molecular Weight48-51 kDa, 32 kDa, 18 kDa
GenBank Accession NumberBC002427
Gene Symbol Caspase 2
Gene ID (NCBI) 835
RRIDAB_2881880
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP42575
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).This antibody can recognize the 32 kDa pro-caspase 2 as well as 18 kDa cleaved-caspase 2.

实验方案

Product Specific Protocols
WB protocol for Caspase 2/p32/p18 antibody 66517-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols