Caspase 2/p32/p18 Monoclonal antibody
Caspase 2/p32/p18 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2G4C2
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy Caspase 2 Ready-To-Use IHC Kit. Caspase 2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66517-1-Ig targets Caspase 2/p32/p18 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human |
| Immunogen | Caspase 2/p32/p18 fusion protein Ag20141 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | caspase 2, apoptosis-related cysteine peptidase |
| Synonyms | CASP 2, CASP2, Caspase 2, Caspase 2/p32/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1 |
| Calculated Molecular Weight | 452 aa, 51 kDa |
| Observed Molecular Weight | 48-51 kDa, 32 kDa, 18 kDa |
| GenBank Accession Number | BC002427 |
| Gene Symbol | Caspase 2 |
| Gene ID (NCBI) | 835 |
| RRID | AB_2881880 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P42575 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).This antibody can recognize the 32 kDa pro-caspase 2 as well as 18 kDa cleaved-caspase 2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 2/p32/p18 antibody 66517-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |