验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy Caspase 2 Ready-To-Use IHC Kit. Caspase 2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66517-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66517 targets Caspase 2/P32/P18 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | Caspase 2/P32/P18 fusion protein Ag20141 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | caspase 2, apoptosis-related cysteine peptidase |
| 别名 | CASP2, Caspase 2, 2G4C2, CASP 2, CASP-2 |
| 计算分子量 | 452 aa, 51 kDa |
| 观测分子量 | 48-51 kDa, 32 kDa, 18 kDa |
| GenBank蛋白编号 | BC002427 |
| 基因名称 | Caspase 2 |
| Gene ID (NCBI) | 835 |
| RRID | AB_2934473 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P42575 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).This antibody can recognize the 32 kDa pro-caspase 2 as well as 18 kDa cleaved-caspase 2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Caspase 2/P32/P18 antibody CL488-66517 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |