验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy Caspase 2 Ready-To-Use IHC Kit. Caspase 2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66517-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66517 targets Caspase 2/p32/p18 in IF/ICC applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human |
| Immunogen | Caspase 2/p32/p18 fusion protein Ag20141 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | caspase 2, apoptosis-related cysteine peptidase |
| Synonyms | CASP 2, CASP2, Caspase 2, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1 |
| Calculated Molecular Weight | 452 aa, 51 kDa |
| Observed Molecular Weight | 48-51 kDa, 32 kDa, 18 kDa |
| GenBank Accession Number | BC002427 |
| Gene Symbol | Caspase 2 |
| Gene ID (NCBI) | 835 |
| RRID | AB_2934473 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P42575 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).This antibody can recognize the 32 kDa pro-caspase 2 as well as 18 kDa cleaved-caspase 2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Caspase 2/p32/p18 antibody CL488-66517 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |