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验证数据展示

  • IF Staining of HeLa using CL488-67398

    IF Staining of HeLa using CL488-67398

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Caspase 4 antibody (CL488-67398, Clone: 1D1E12 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Caspase 4 Monoclonal antibody

Caspase 4 Monoclonal Antibody for IF/ICC
Cat No. CL488-67398

Print datasheet

CloneNo. 1D1E12

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

CASP 4, CASP4, Caspase 4, Caspase4, ICE(rel) II, ICE(rel)II, ICEREL II, ICH 2, ICH2, Mih1/TX, Protease ICH 2, Protease TX, TX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67398-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67398 targets Caspase 4 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen Caspase 4 fusion protein Ag29488 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caspase 4, apoptosis-related cysteine peptidase
Synonyms CASP 4, CASP4, Caspase 4, Caspase4, ICE(rel) II, ICE(rel)II, ICEREL II, ICH 2, ICH2, Mih1/TX, Protease ICH 2, Protease TX, TX
Calculated Molecular Weight 377 aa, 43 kDa
Observed Molecular Weight 43-48 kDa
GenBank Accession NumberBC017839
Gene Symbol Caspase 4
Gene ID (NCBI) 837
RRIDAB_2934542
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49662
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Caspase 4 antibody CL488-67398Download protocol
Standard Protocols
Click here to view our Standard Protocols