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Caspase 6 Monoclonal antibody

Caspase 6 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67825-1-Ig

产品说明书

CloneNo. 1H7F9

宿主/亚型

Mouse / IgG2b

种属反应性

Human and More (1)

应用

WB, IF/ICC, ELISA

Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67825-1-Ig targets Caspase 6 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human
免疫原 Caspase 6 fusion protein Ag20534 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase 6, apoptosis-related cysteine peptidase
别名 Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2
计算分子量 33 kDa, 22 kDa
观测分子量 33-35 kDa,22kDa, 18kDa
GenBank蛋白编号BC000305
基因名称 Caspase 6
Gene ID (NCBI) 839
RRIDAB_2918587
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55212
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).

实验方案

Product Specific Protocols
WB protocol for Caspase 6 antibody 67825-1-IgDownload protocol
IF protocol for Caspase 6 antibody 67825-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Caspase-mediated AURKA cleavage at Asp132 is essential for paclitaxel to elicit cell apoptosis

Authors - Xiaoting Chen
  • KO Validated
humanWB

Apoptosis

Strong activation of p53 by actinomycin D and nutlin-3a overcomes the resistance of cancer cells to the pro-apoptotic activity of the FAS ligand

Authors - Barbara Łasut-Szyszka
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