验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells |
Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 2 publications below |
产品信息
67825-1-Ig targets Caspase 6 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human |
Cited Reactivity | human |
Immunogen | Caspase 6 fusion protein Ag20534 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | caspase 6, apoptosis-related cysteine peptidase |
Synonyms | Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2 |
Calculated Molecular Weight | 33 kDa, 22 kDa |
Observed Molecular Weight | 33-35 kDa,22kDa, 18kDa |
GenBank Accession Number | BC000305 |
Gene Symbol | Caspase 6 |
Gene ID (NCBI) | 839 |
RRID | AB_2918587 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P55212 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).
实验方案
Product Specific Protocols | |
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WB protocol for Caspase 6 antibody 67825-1-Ig | Download protocol |
IF protocol for Caspase 6 antibody 67825-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Theranostics Caspase-mediated AURKA cleavage at Asp132 is essential for paclitaxel to elicit cell apoptosis
| ||
Apoptosis Strong activation of p53 by actinomycin D and nutlin-3a overcomes the resistance of cancer cells to the pro-apoptotic activity of the FAS ligand |