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  • Featured Product
  • KD/KO Validated

Caspase 6 Monoclonal antibody, PBS Only

Caspase 6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H7F9

Cat no : 67825-1-PBS

Print datasheet

Synonyms

Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2

验证数据展示

  • WB analysis using 67825-1-Ig (same clone as 67825-1-PBS)

    WB analysis using 67825-1-Ig (same clone as 67825-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67825-1-Ig (Caspase 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    WB analysis of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    WB result of Caspase 6 antibody (67825-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 6 transfected Jurkat cells. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    IF Staining of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Caspase 6 antibody (67825-1-Ig, Clone: 1H7F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

产品信息

67825-1-PBS targets Caspase 6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Caspase 6 fusion protein Ag20534 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name caspase 6, apoptosis-related cysteine peptidase
Synonyms Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2
Calculated Molecular Weight 33 kDa, 22 kDa
Observed Molecular Weight 33-35 kDa，22kDa, 18kDa
GenBank Accession NumberBC000305
Gene Symbol Caspase 6
Gene ID (NCBI) 839
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55212
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).