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验证数据展示

  • WB analysis using 67825-1-Ig (same clone as 67825-1-PBS)

    WB analysis using 67825-1-Ig (same clone as 67825-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67825-1-Ig (Caspase 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    WB analysis of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    WB result of Caspase 6 antibody (67825-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 6 transfected Jurkat cells. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    IF Staining of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Caspase 6 antibody (67825-1-Ig, Clone: 1H7F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Caspase 6 Monoclonal antibody, PBS Only

Caspase 6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67825-1-PBS

产品说明书

CloneNo. 1H7F9

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IF/ICC, Indirect ELISA

别名

Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67825-1-PBS targets Caspase 6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 Caspase 6 fusion protein Ag20534 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase 6, apoptosis-related cysteine peptidase
别名 Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2
计算分子量 33 kDa, 22 kDa
观测分子量 33-35 kDa，22kDa, 18kDa
GenBank蛋白编号BC000305
基因名称 Caspase 6
Gene ID (NCBI) 839
RRIDAB_2918587
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55212
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).