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Caspase 7 Monoclonal antibody, PBS Only

Caspase 7 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C9H4

Cat no : 67956-1-PBS

Print datasheet

Synonyms

Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3

验证数据展示

  • WB analysis using 67956-1-Ig (same clone as 67956-1-PBS)

    WB analysis using 67956-1-Ig (same clone as 67956-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67956-1-Ig (same clone as 67956-1-PBS)

    IHC staining of human liver cancer using 67956-1-Ig (same clone as 67956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASP7 antibody included
  • IHC staining of human liver cancer using 67956-1-Ig (same clone as 67956-1-PBS)

    IHC staining of human liver cancer using 67956-1-Ig (same clone as 67956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASP7 antibody included
  • IHC staining of mouse liver using 67956-1-Ig (same clone as 67956-1-PBS)

    IHC staining of mouse liver using 67956-1-Ig (same clone as 67956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASP7 antibody included
  • IHC staining of mouse stomach using 67956-1-Ig (same clone as 67956-1-PBS)

    IHC staining of mouse stomach using 67956-1-Ig (same clone as 67956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASP7 antibody included
  • IHC staining of rat liver using 67956-1-Ig (same clone as 67956-1-PBS)

    IHC staining of rat liver using 67956-1-Ig (same clone as 67956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASP7 antibody included
  • IHC staining of rat stomach using 67956-1-Ig (same clone as 67956-1-PBS)

    IHC staining of rat stomach using 67956-1-Ig (same clone as 67956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASP7 antibody included
  • IHC staining of rat stomach using 67956-1-Ig (same clone as 67956-1-PBS)

    IHC staining of rat stomach using 67956-1-Ig (same clone as 67956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASP7 antibody included
Planning an IHC experiment? We recommend our IHCeasy CASP7 Ready-To-Use IHC Kit. CASP7 primary antibody included.

产品信息

67956-1-PBS targets Caspase 7 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Caspase 7 fusion protein Ag27601 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name caspase 7, apoptosis-related cysteine peptidase
Synonyms Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3
Calculated Molecular Weight 303 aa, 34 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC015799
Gene Symbol Caspase 7
Gene ID (NCBI) 840
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55210
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.