IHCeasy CASP7 Ready-To-Use IHC Kit
CASP7 Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC0406
Synonyms
Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3
Validation Data Gallery
Product Information
KHC0406 is a ready-to-use IHC kit for staining of CASP7. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy CASP7 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, proform is converted into a intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large subunit and the p10 or small subunit and it is present in the brain, which is up-regulated and activated after traumatic injury. Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9. The protein is involved in the activation cascade of caspases responsible for apoptosis execution.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3 |
