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Catalase Monoclonal antibody

Catalase Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66765-1-Ig

产品说明书

CloneNo. 1F8D7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

CAT, EC:1.11.1.6, 1F8D7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, rat brain tissue, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66765-1-Ig targets Catalase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat, pig, goat
免疫原 Catalase fusion protein Ag16916 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 catalase
别名 CAT, EC:1.11.1.6, 1F8D7
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC112219
基因名称 Catalase
Gene ID (NCBI) 847
RRIDAB_2882111
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04040
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.

实验方案

Product Specific Protocols
WB protocol for Catalase antibody 66765-1-IgDownload protocol
IHC protocol for Catalase antibody 66765-1-IgDownload protocol
IF protocol for Catalase antibody 66765-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Microbiome

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.

Authors - Leli Wang
mouseWB

Biomaterials

MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.

Authors - Tian Wang
humanIF

Elife

Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stress

Authors - Jinyang Wang
goatWB

Oxid Med Cell Longev

Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.

Authors - Tian-Yu Feng
ratWB

Oxid Med Cell Longev

Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis.

Authors - Honglin Liu
mouseWB

Antioxidants (Basel)

High-Intensity Interval Training and Moderate-Intensity Continuous Training Attenuate Oxidative Damage and Promote Myokine Response in the Skeletal Muscle of ApoE KO Mice on High-Fat Diet.

Authors - Linjia Wang
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