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  • Featured Product
  • KD/KO Validated

Catalase Monoclonal antibody, PBS Only

Catalase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F8D7

Cat no : 66765-1-PBS

Print datasheet

Synonyms

CAT, EC:1.11.1.6, 1F8D7

验证数据展示

  • WB analysis using 66765-1-Ig (same clone as 66765-1-PBS)

    WB analysis using 66765-1-Ig (same clone as 66765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66765-1-Ig (same clone as 66765-1-PBS)

    WB analysis of HeLa using 66765-1-Ig (same clone as 66765-1-PBS)

    WB result of Catalase antibody (66765-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Catalase transfected HeLa cells. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66765-1-Ig (same clone as 66765-1-PBS)

    IF Staining of HepG2 using 66765-1-Ig (same clone as 66765-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Catalase antibody (66765-1-Ig, Clone: 1F8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocirrhosis using 66765-1-Ig (same clone as 66765-1-PBS)

    IHC staining of human hepatocirrhosis using 66765-1-Ig (same clone as 66765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocirrhosis using 66765-1-Ig (same clone as 66765-1-PBS)

    IHC staining of human hepatocirrhosis using 66765-1-Ig (same clone as 66765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66765-1-Ig (same clone as 66765-1-PBS)

    IF Staining of HepG2 using 66765-1-Ig (same clone as 66765-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66765-1-Ig (Catalase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • WB analysis using 66765-1-Ig (same clone as 66765-1-PBS)

    WB analysis using 66765-1-Ig (same clone as 66765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

产品信息

66765-1-PBS targets Catalase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Catalase fusion protein Ag16916 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name catalase
Synonyms CAT, EC:1.11.1.6, 1F8D7
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC112219
Gene Symbol Catalase
Gene ID (NCBI) 847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04040
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.