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验证数据展示

  • WB analysis using 83743-2-RR (same clone as 83743-2-PBS)

    WB analysis using 83743-2-RR (same clone as 83743-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

    WB analysis of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00695-4

    Sandwich ELISA standard curve of MP00695-4

    Sandwich ELISA standard curve of MP00695-4, Human Cathepsin D Recombinant Matched Antibody Pair - PBS only. 83743-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1053. 83743-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP00695-4

    Sample test of MP00695-4

    Saliva of eight individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 139.6 ng/mL with a range of 102.4 - 182.3 ng/mL.

  • Sample test of MP00695-4

    Sample test of MP00695-4

    Human milk of eight individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 580.4 ng/mL with a range of 270 - 1,071.8 ng/mL.

  • Sample test of MP00695-4

    Sample test of MP00695-4

    For the A431 supernatant, the mean human Cathepsin D concentration was determined to be 301.05 ng/mL.

  • Sample test of MP00695-4

    Sample test of MP00695-4

    Serum of sixteen individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 147.3 ng/mL with a range of 43.2 - 224.2 ng/mL.

  • IF Staining of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

    IF Staining of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (83743-2-RR, Clone: 240775E7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FLNA antibody (67133-1-Ig, Clone: 1G4H3, red). This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

    FC experiment of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cathepsin D Recombinant antibody (83743-2-RR, Clone:240775E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00695-1

    Cytometric bead array standard curve of MP00695-1

    Cytometric bead array standard curve of MP00695-1, Cathepsin D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83743-1-PBS. Detection antibody: 83743-2-PBS. Standard: Eg1053. Range: 0.78-100 ng/mL.

Cathepsin D Recombinant antibody, PBS Only (Detector)

Cathepsin D Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83743-2-PBS

产品说明书

CloneNo. 240775E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Cathepsin D Ready-To-Use IHC Kit. Cathepsin D primary antibody included.

产品信息

83743-2-PBS targets Cathepsin D as part of a matched antibody pair:

MP00695-1: 83743-1-PBS capture and 83743-2-PBS detection (validated in Cytometric bead array)

MP00695-4: 83743-6-PBS capture and 83743-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cathepsin D
别名 CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain
计算分子量45kDa
观测分子量 32 kDa, 48 kDa, 52 kDa
GenBank蛋白编号NM_001909.5
基因名称 Cathepsin D
Gene ID (NCBI) 1509
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07339
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).