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验证数据展示

  • WB analysis using 83743-2-RR

    WB analysis using 83743-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 83743-2-RR

    WB analysis of MCF-7 using 83743-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 83743-2-RR

    IF Staining of MCF-7 using 83743-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (83743-2-RR, Clone: 240775E7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FLNA antibody (67133-1-Ig, Clone: 1G4H3, red).

  • FC experiment of MCF-7 using 83743-2-RR

    FC experiment of MCF-7 using 83743-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cathepsin D Recombinant antibody (83743-2-RR, Clone:240775E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Cathepsin D Recombinant antibody

Cathepsin D Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83743-2-RR

Print datasheet

CloneNo. 240775E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells, SH-SY5Y cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Cathepsin D Ready-To-Use IHC Kit. Cathepsin D primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83743-2-RR targets Cathepsin D in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cathepsin D
Synonyms CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain
Calculated Molecular Weight45kDa
Observed Molecular Weight 32 kDa, 48 kDa, 52 kDa
GenBank Accession NumberNM_001909.5
Gene Symbol Cathepsin D
Gene ID (NCBI) 1509
RRIDAB_3671341
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07339
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).

实验方案

Product Specific Protocols
WB protocol for Cathepsin D antibody 83743-2-RRDownload protocol
IF protocol for Cathepsin D antibody 83743-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols