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验证数据展示

  • IF Staining of HeLa using CL594-66914

    IF Staining of HeLa using CL594-66914

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Cathepsin L antibody (CL594-66914, Clone: 1A12G8 ) at dilution of 1:200.

  • FC experiment of A549 using CL594-66914

    FC experiment of A549 using CL594-66914

    1X10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Cathepsin L (CL594-66914, Clone:1A12G8) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Cathepsin L Monoclonal antibody

Cathepsin L Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66914

产品说明书

CloneNo. 1A12G8

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

CTSL1, CATL, cathepsinL1, cathepsin L1, Cathepsin L light chain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy CTSL1 Ready-To-Use IHC Kit. CTSL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66914-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66914 targets Cathepsin L in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Cathepsin L fusion protein Ag27951 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cathepsin L1
别名 CTSL1, CATL, cathepsinL1, cathepsin L1, Cathepsin L light chain
计算分子量 38 kDa
观测分子量43 kDa
GenBank蛋白编号BC012612
基因名称 Cathepsin L
Gene ID (NCBI) 1514
RRIDAB_3084735
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07711
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

实验方案

Product Specific Protocols
IF protocol for CL594 Cathepsin L antibody CL594-66914Download protocol
FC protocol for CL594 Cathepsin L antibody CL594-66914Download protocol
Standard Protocols
Click here to view our Standard Protocols