• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Cathepsin L Monoclonal antibody

Cathepsin L Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A12G8

Cat no : CL594-66914

Print datasheet

Synonyms

CTSL1, CATL, cathepsinL1, cathepsin L1, Cathepsin L light chain

验证数据展示

  • IF Staining of HeLa using CL594-66914

    IF Staining of HeLa using CL594-66914

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Cathepsin L antibody (CL594-66914, Clone: 1A12G8 ) at dilution of 1:200.

  • FC experiment of A549 using CL594-66914

    FC experiment of A549 using CL594-66914

    1X10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Cathepsin L (CL594-66914, Clone:1A12G8) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy CTSL1 Ready-To-Use IHC Kit. CTSL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66914-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66914 targets Cathepsin L in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen Cathepsin L fusion protein Ag27951 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cathepsin L1
Synonyms CTSL1, CATL, cathepsinL1, cathepsin L1, Cathepsin L light chain
Calculated Molecular Weight 38 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC012612
Gene Symbol Cathepsin L
Gene ID (NCBI) 1514
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07711
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

实验方案

Product Specific Protocols
IF protocol for CL594 Cathepsin L antibody CL594-66914Download protocol
FC protocol for CL594 Cathepsin L antibody CL594-66914Download protocol
Standard Protocols
Click here to view our Standard Protocols