• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

    WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67631-1-Ig (same clone as 67631-1-PBS)

    IF Staining of HepG2 using 67631-1-Ig (same clone as 67631-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ch-TOG antibody (67631-1-Ig, Clone: 1H5G1 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 67631-1-Ig (same clone as 67631-1-PBS)

    IHC staining of mouse cerebellum using 67631-1-Ig (same clone as 67631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKAP5/Ch-TOG antibody included
  • IHC staining of mouse cerebellum using 67631-1-Ig (same clone as 67631-1-PBS)

    IHC staining of mouse cerebellum using 67631-1-Ig (same clone as 67631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKAP5/Ch-TOG antibody included
  • IHC staining of human rectal cancer using 67631-1-Ig (same clone as 67631-1-PBS)

    IHC staining of human rectal cancer using 67631-1-Ig (same clone as 67631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKAP5/Ch-TOG antibody included
  • IHC staining of human rectal cancer using 67631-1-Ig (same clone as 67631-1-PBS)

    IHC staining of human rectal cancer using 67631-1-Ig (same clone as 67631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKAP5/Ch-TOG antibody included
  • Cytometric bead array standard curve of MP50224-1

    Cytometric bead array standard curve of MP50224-1

    Cytometric bead array standard curve of MP50224-1, CKAP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67631-2-PBS. Detection antibody: 67631-1-PBS. Standard:Ag28770. Range: 0.391-100 ng/mL.

  • Cytometric bead array standard curve of MP50224-2

    Cytometric bead array standard curve of MP50224-2

    Cytometric bead array standard curve of MP50224-2, CKAP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67631-1-PBS. Detection antibody: 67631-3-PBS. Standard:Ag28770. Range: 6.25-100 ng/mL.

Ch-TOG Monoclonal antibody, PBS Only (Capture/Detector)

Ch-TOG Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67631-1-PBS

产品说明书

CloneNo. 1H5G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

CKAP5, TOGp, Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, ch TOG

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CKAP5/Ch-TOG Ready-To-Use IHC Kit. CKAP5/Ch-TOG primary antibody included.

产品信息

67631-1-PBS targets Ch-TOG as part of a matched antibody pair:

MP50224-1: 67631-2-PBS capture and 67631-1-PBS detection (validated in Cytometric bead array)

MP50224-2: 67631-1-PBS capture and 67631-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Ch-TOG fusion protein Ag28770 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cytoskeleton associated protein 5
别名 CKAP5, TOGp, Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, ch TOG
观测分子量 220-240 kDa
GenBank蛋白编号BC120869
基因名称 Ch-TOG
Gene ID (NCBI) 9793
RRIDAB_2882832
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14008
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.