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验证数据展示

  • WB analysis using 67631-1-Ig

    WB analysis using 67631-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67631-1-Ig

    IF Staining of HepG2 using 67631-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ch-TOG antibody (67631-1-Ig, Clone: 1H5G1 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of mouse cerebellum using 67631-1-Ig

    IHC staining of mouse cerebellum using 67631-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CKAP5/Ch-TOG antibody included
  • IHC staining of mouse cerebellum using 67631-1-Ig

    IHC staining of mouse cerebellum using 67631-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CKAP5/Ch-TOG antibody included
  • IHC staining of human rectal cancer using 67631-1-Ig

    IHC staining of human rectal cancer using 67631-1-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CKAP5/Ch-TOG antibody included
  • IHC staining of human rectal cancer using 67631-1-Ig

    IHC staining of human rectal cancer using 67631-1-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CKAP5/Ch-TOG antibody included

Ch-TOG Monoclonal antibody

Ch-TOG Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67631-1-Ig
CloneNo. 1H5G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CKAP5, TOGp, Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, ch TOG

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, HeLa cells, HepG2 cells, NCCIT cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse cerebellum tissue, human rectal cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CKAP5/Ch-TOG Ready-To-Use IHC Kit. CKAP5/Ch-TOG primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67631-1-Ig targets Ch-TOG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Ch-TOG fusion protein Ag28770 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytoskeleton associated protein 5
Synonyms CKAP5, TOGp, Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, ch TOG
Observed Molecular Weight 220-240 kDa
GenBank Accession NumberBC120869
Gene Symbol Ch-TOG
Gene ID (NCBI) 9793
RRIDAB_2882832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14008
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.

实验方案

Product Specific Protocols
WB protocol for Ch-TOG antibody 67631-1-IgDownload protocol
IHC protocol for Ch-TOG antibody 67631-1-IgDownload protocol
IF protocol for Ch-TOG antibody 67631-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols