验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CKAP5/Ch-TOG Ready-To-Use IHC Kit. CKAP5/Ch-TOG primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67631-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-67631 targets Ch-TOG in IF/ICC applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, mouse, rat, pig |
| 免疫原 | Ch-TOG fusion protein Ag28770 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cytoskeleton associated protein 5 |
| 别名 | ch TOG, CHTOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp |
| 观测分子量 | 220-240 kDa |
| GenBank蛋白编号 | BC120869 |
| 基因名称 | Ch-TOG |
| Gene ID (NCBI) | 9793 |
| RRID | AB_2919511 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q14008 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Ch-TOG antibody CL488-67631 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |