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CoraLite®594-conjugated Ch-TOG Monoclonal antibody

Ch-TOG Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H5G1

Cat no : CL594-67631

Print datasheet

Synonyms

ch TOG, CHTOG, Ch-TOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp

验证数据展示

  • IF Staining of HepG2 using CL594-67631

    IF Staining of HepG2 using CL594-67631

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Ch-TOG antibody (CL594-67631, Clone: 1H5G1 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CKAP5/Ch-TOG Ready-To-Use IHC Kit. CKAP5/Ch-TOG primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67631-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67631 targets Ch-TOG in IF/ICC applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen Ch-TOG fusion protein Ag28770 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytoskeleton associated protein 5
Synonyms ch TOG, CHTOG, Ch-TOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp
Observed Molecular Weight 220-240 kDa
GenBank Accession NumberBC120869
Gene Symbol Ch-TOG
Gene ID (NCBI) 9793
RRIDAB_2920166
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14008
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.

实验方案

Product Specific Protocols
IF protocol for CL594 Ch-TOG antibody CL594-67631Download protocol
Standard Protocols
Click here to view our Standard Protocols