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Chicken IgY Monoclonal antibody

Chicken IgY Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Chicken

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E12G2

Cat no : 67405-1-Ig

Print datasheet

Synonyms

验证数据展示

  • WB analysis of Recombinant protein using 67405-1-Ig

    WB analysis of Recombinant protein using 67405-1-Ig

    100 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

经过测试的应用

Positive WB detected inChicken IgY, Chicken egg

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67405-1-Ig targets Chicken IgY in WB, ELISA applications and shows reactivity with Chicken samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Chicken IgY
Synonyms
Observed Molecular Weight 180 kDa, 70 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2882646
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgY (immunoglobulin Y) is a type of immunoglobulin found in birds and reptiles. High concentration of IgY exists in chicken egg yolk. In chickens, IgY is functional same to IgG. IgY antibodies do not cross-react with Fc receptors, mammalian immunoglobulins such as rheumatoid factors and HAMA or activate the complement system thus could reduce false positive results in immunological assays. This antibody was generated from chicken IgY that extracted from chicken eggs. This product recognizes the heavry chain of checken IgY and can detect the contact form of chicken IgY (~180 kDa) and reduced form (~70 kDa).

实验方案

Product Specific Protocols
WB protocol for Chicken IgY antibody 67405-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Carbohydr Polym

Carboxymethyl chitosan assembled piezoelectric biosensor for rapid and label-free quantification of immunoglobulin Y.

Authors - Hao Li
View Article